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KMID : 0900919970210020117
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Korean journal of Animal Reproduction
1997 Volume.21 No. 2 p.117 ~ p.122
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Oocytes Maturation by Follicular Culture in Porcine
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Park Choon-Keun
Lee Joon-Hee
Lee Sang-Young
Cheong Hee-Tae
Yang Boo-Keun
Kim Choung-Ik
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Abstract
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The aim of this study was to investigate the effect of the follicular culture from which the oocytes originate on their subsequent in vitro maturation ability. Ovarian follicles were isolated and cultured according to size(1~2mm, 2~6mm and 6~8mm) for 42~44 h. The rates of germinal vesicle breakdown(GVBD) in each groups were 87%(65/75), 82%(80/97) and 89%(47/53), but the oocytes maturation were su, pp.essed at anaphase-I stage. In spite of the adding porcine follicular fluid and/or hormones in maturation medium, maturation ability of oocytes from follicle cultured for 21~22 h were inhibited. When oocytes from follicle cultured for 4 h at various temperature were incubated for 38~40 h, the rates of oocytes maturation from follicle cultured at 20^{circ}C(51%, 26/51) and 39^{circ}C(54%, 26/48) were significant higher(P<0.05) than group cultured at 4^{circ}C(33%, 19/58). On the other hand, the GVBD were stared 2 h after culture of follicle of oocytes. To summairze, oocytes maturation by follicular culture were inhibited at anaphase-I stage in porcine. When the follicle cultured for 4 h, maturation were completed to metaphase-II stage. However, rates of GVBD in oocytes from follicular culture were higher than oocytes cultured in medium.
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KEYWORD
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Follicular culture, Oocytes maturation, Germinal vesicle breakdown, Porcine
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